Open AccessNew osmoregulated beta(1-3),beta(1-6) glucosyltransferase(s) in Azospirillum brasilense
Author(s) -
Silvia Altabe,
Nora Iñón de Iannino,
Diego de Mendoza,
Rodolfo A. Ugalde
Publication year - 1994
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.176.16.4890-4898.1994
Subject(s) - periplasmic space , azospirillum brasilense , biology , glucosyltransferase , beta (programming language) , biochemistry , glucan , microbiology and biotechnology , enzyme , beta glucan , bacteria , escherichia coli , microbial inoculant , genetics , computer science , programming language , gene
A linear beta(1-3),beta(1-6) glucan was detected in the periplasm of Azospirillum brasilense cells growing in a medium of low osmotic strength. This glucan was produced in vitro by purified bacterial inner membranes with UDP-glucose as the sugar donor in the presence of Mg2+. Growth in a high-osmotic-strength medium strongly reduced the amount of this glucan accumulated in the periplasmic space, and the inhibition was associated with a reduction in the enzymatic activity of the beta(1-3),beta(1-6) glucosyltransferase(s).