Open AccessA Novel Insertion Sequence Derepresses Efflux Pump Expression and Preadapts Pseudomonas putida S12 for Extreme Solvent Stress
Author(s) -
Xinwei Sun,
Jonathan J. Dennis
Publication year - 2009
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.00832-09
Subject(s) - efflux , pseudomonas putida , biology , repressor , solvent , insertion sequence , sequence (biology) , genetics , microbiology and biotechnology , gene , biochemistry , gene expression , transposable element , mutant
A multidrug efflux pump, SrpABC, plays a key role inPseudomonas putida S12 tolerance to toxic organic solvents. SrpRS are putative regulators of the SrpABC efflux pump encoded upstream of thesrpABC structural genes, and previous studies suggest that SrpS is a repressor of SrpABC expression. An S12 isolate able to withstand extreme solvent stress carries a novel insertion sequence, ISPpu21 , interruptingsrpS . This insertion preadapts S12 to extreme solvent conditions through constitutive SrpABC expression.