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Genes encoding key enzymes of the ectoine biosynthesis pathway in the halotolerant obligate methanotrophMethylomicrobium alcaliphilum 20Z have been shown to be organized into anectABC -ask operon. Transcription of theect operon is initiated from two promoters,ectAp 1 andectAp 2 (ectAp 1 p 2 ), similar to the σ70 -dependent promoters ofEscherichia coli . Upstream of the gene cluster, an open reading frame (ectR1 ) encoding a MarR-like transcriptional regulator was identified. Investigation of the influence of EctR1 on the activity of theectAp 1 p 2 promoters in wild-typeM. alcaliphilum 20Z andectR1 mutant strains suggested that EctR1 is a negative regulator of theectABC -ask operon. Purified recombinant EctR1-His6 specifically binds as a homodimer to the putative −10 motif of theectAp 1 promoter. The EctR1 binding site contains a pseudopalindromic sequence (TATTTAGT-GT-ACTATATA) composed of 8-bp half-sites separated by 2 bp. Transcription of theectR1 gene is initiated from a single σ70 -like promoter. The location of the EctR1 binding site between the transcriptional and translational start sites of theectR1 gene suggests that EctR1 may regulate its own expression. The data presented suggest that inMethylomicrobium alcaliphilum 20Z, EctR1-mediated control of the transcription of theect genes is not the single mechanism for the regulation of ectoine biosynthesis.

journal of bacteriology

Identification and Characterization of EctR1, a New Transcriptional Regulator of the Ectoine Biosynthesis Genes in the Halotolerant Methanotroph <i>Methylomicrobium alcaliphilum</i> 20Z

Identification and Characterization of EctR1, a New Transcriptional Regulator of the Ectoine Biosynthesis Genes in the Halotolerant Methanotroph Methylomicrobium alcaliphilum 20Z