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The complex segmented genome ofBorrelia burgdorferi is comprised of a linear chromosome along with numerous linear and circular plasmids essential for tick and/or mammalian infectivity. The pathogenic necessity for specific borrelial plasmids has been identified; most notably, infections of the tick vector and mammalian host both require linear plasmid 25 (lp25). Genes carried on lp25, specificallybptA andpncA , are postulated to play a role forB. burgdorferi to infect and persist inIxodes ticks. In this study, we complemented an lp25-deficient borrelial strain withpncA alone orpncA accompanied bybptA to evaluate the ability of the complemented strains to restore larval colonization and persistence through transstadial transmission relative to that of wild-typeB. burgdorferi . The acquisition of the complemented strains by tick larvae from infected mice and/or the survival of these strains was significantly decreased when assayed by cultivation and quantitative PCR (qPCR). Only 10% of thepncA -complemented strain organisms were found by culture to survive 17 days following larval feeding, while 45% of thepncA - andbptA -complemented strain organisms survived, with similar results by PCR. However, neither of the complementedB. burgdorferi strains was capable of persisting through the molt to the nymphal stage as analyzed by culture. qPCR analyses of unfed nymphs detectedB. burgdorferi genomes in several nymphs at low copy numbers, likely indicating the presence of DNA from dead or dying cells. Overall, the data indicate thatpncA andbptA cannot independently support infection, suggesting that lp25 carries additional gene(s) or regulatory elements critical forB. burgdorferi survival and pathogenesis in theIxodes vector.

infection and immunity

<i>pncA</i> and <i>bptA</i> Are Not Sufficient To Complement Ixodes scapularis Colonization and Persistence by Borrelia burgdorferi in a Linear Plasmid lp25-Deficient Background

pncA and bptA Are Not Sufficient To Complement Ixodes scapularis Colonization and Persistence by Borrelia burgdorferi in a Linear Plasmid lp25-Deficient Background