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A gene cassette incorporating the crs-rsd site-specific recombination system from the Salmonella enterica subsp. enterica serovar Dublin virulence plasmid improved the inheritance in S. enterica serotype Typhi strain CVD908-htrA of a multicopy plasmid expression vector. Use of this recombination cassette may improve expression of heterologous antigens from multicopy plasmid expression vectors in attenuated bacterial vaccine strains.

infection and immunity

Stabilization of a Plasmid Coding for a Heterologous Antigen in <i>Salmonella enterica</i> Serotype Typhi Vaccine Strain CVD908- <i>htrA</i> by Using Site-Specific Recombination

Stabilization of a Plasmid Coding for a Heterologous Antigen in Salmonella enterica Serotype Typhi Vaccine Strain CVD908- htrA by Using Site-Specific Recombination