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The genome sequence of the entomopathogen  Bacillus thuringiensis  subsp.  thuringiensis  strain IS5056 was determined. The chromosome is composed of 5,491,935 bp. In addition, IS5056 harbors 14 plasmids ranging from 6,880 to 328,151 bp, four of which contain nine insecticidal protein genes,  cry1Aa3 ,  cry1Ab21 ,  cry1Ba1 ,  cry1Ia14 ,  cry2Aa9 ,  cry2Ab1 ,  vip1 ,  vip2 , and  vip3Aa10 .

Complete Genome Sequence of Bacillus thuringiensis subsp. thuringiensis Strain IS5056, an Isolate Highly Toxic to Trichoplusia ni