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We announce the availability of the 5.023-Mbp high-quality draft assembly of the  Escherichia coli  strain Nissle 1917 (serovar O6:K5:H1) genome. Short genomic segments from this important probiotic strain have been available in public databases, but the full genome sequence has remained inaccessible. Thus, high-coverage, whole genome sequencing of  E. coli  Nissle 1917 is presented herein. Reannotation and metabolic reconstruction will enable comparative genomics analysis and model-guided predictions of genetic manipulations leading to increased production of the K5 capsular polysaccharide known as  N -acetyl heparosan, a precursor to the anticoagulant pharmaceutical heparin.

Draft Genome Sequence of Escherichia coli Strain Nissle 1917 (Serovar O6:K5:H1)