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The 1,670-bp nucleotide sequence of the heat shock operon groESL and the 1,236-bp sequence of the citrate synthase gene (gltA) of Anaplasma (Ehrlichia) platys were determined. The topology of the groEL- and gltA-based phylogenetic tree was similar to that derived from 16S rRNA gene analyses with distances. Both groESL- and gltA-based PCRs specific to A. platys were also developed based upon the alignment data.

clinical and vaccine immunology

Determination of the Nucleotide Sequences of Heat Shock Operon groESL and the Citrate Synthase Gene ( gltA ) of Anaplasma ( Ehrlichia ) platys for Phylogenetic and Diagnostic Studies