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A study has been made to revaluate to what extent the coliform group and the enterococci are present in soil, which is apparently undisturbed soil, using modern techniques for their isolation and identificationi. Three hundred and sixty-nine soil samples were takeit from areas adjacent to four Western Massachusetts water supply reservoirs in which past fecal contamination was either nonexistent or very remote due to the sanitary control of the watersheds. Two hundred and seventy samples (73.4 per cent) conitained coliform bacteria, as indicated by the presence of gas in lactose broth seeded from eosin methylene blue agar plates, and/or presence of gas in brilliant green bile broth. Five samples (1.4 per cent) yielded typical Escherichia coli colonies on eosin methylene blue agar. Enterococci were found in 8 (2.2 per cent) of the :369 soil samples examined.

Nutrition and the Development of Mushroom Flavor in Agaricus campestris Mycelium