Open AccessDiscovery of glpC , an Organic Solvent Tolerance-Related Gene in Escherichia coli , Using Gene Expression Profiles from DNA Microarrays
Author(s) -
Kazunori Shimizu,
Shogo Hayashi,
Takeshi Kako,
Maiko Suzuki,
Norihiko Tsukagoshi,
Noriyuki Doukyu,
Takeshi Kobayashi,
Hiroyuki Honda
Publication year - 2005
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.71.2.1093-1096.2005
Subject(s) - escherichia coli , gene , gene expression , biology , dna microarray , dna , microbiology and biotechnology , biochemistry , genetics
Gene expression profiles were collected from Escherichia coli strains (OST3410, TK33, and TK31) before and after exposure to organic solvents, and the six genes that showed higher gene expression were selected. Among these genes, glpC encoding the anaerobic glycerol-3-phosphate dehydrogenase subunit C remarkably increased the organic solvent tolerance.