Open AccessMolecular Forensic Profiling of Cryptosporidium Species and Genotypes in Raw Water
Author(s) -
Norma J. Ruecker,
Niravanh Bounsombath,
P. M. Wallis,
Corinne S. L. Ong,
Judith L. Isaac-Renton,
Norman F. Neumann
Publication year - 2005
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.71.12.8991-8994.2005
Subject(s) - cryptosporidium , biology , genotype , dna profiling , restriction fragment length polymorphism , feces , computational biology , dna , microbiology and biotechnology , genetics , gene
The emerging concept of host specificity ofCryptosporidium spp. was exploited to characterize sources of fecal contamination in a watershed. A method of molecular forensic profiling ofCryptosporidium oocysts on microscope slides prepared from raw water samples processed by U.S. Environmental Protection Agency Method 1623 was developed. The method was based on a repetitive nested PCR-restriction fragment length polymorphism-DNA sequencing approach that permitted the resolution of multiple species/genotypes ofCryptosporidium in a single water sample.