Open AccessFluorogenic Selective and Differential Medium for Isolation of Enterobacter sakazakii
Author(s) -
SeWook Oh,
DongHyun Kang
Publication year - 2004
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.70.9.5692-5694.2004
Subject(s) - enterobacter , bacteria , microorganism , isolation (microbiology) , microbiology and biotechnology , biology , substrate (aquarium) , incubation , growth medium , enterobacteriaceae , chemistry , chromatography , escherichia coli , biochemistry , gene , genetics , ecology
4-Methylumbelliferyl-alpha-D-glucoside, the fluorogenic substrate of alpha-glucosidase, was used as a selective marker to develop a differential medium for Enterobacter sakazakii. This bacterium showed strong fluorogenic characteristics clearly distinguishable from other microorganisms. On the basis of reducing background noise, an optimum basal medium and nitrogen source were selected. Incubation conditions were optimized.