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new technique for its use as an inhibitive agent. Azide in a medium gives rise to hydrazoic acid which volatilizes, diffuses through the air in a closed vessel such as a Petri dish or jar, and may be absorbed by medium that is not in direct contact with the azidecontaining medium so as to produce characteristic inhibition. Spot tests for azide (hydrazoic acid) (Feigl, 1943) may be obtained with the atmosphere of the container or with medium in contact with the atmosphere of the container. Hydrazoic acid is liberated from uninoculated as well as inoculated medium. Although hydrazoic acid has a boiling point of 37 C, it is apparently liberated from the medium as readily at 30 C as at 37 C. More hydrazoic acid is liberated from an acid medium, although Nwe found some to be liberated from a medium at pH 7.6, the most alkaline medium that we tested. These observations suggested that incubation of a medium in a jar containing an atmosphere of hydrazoic acid might replace the direct addition of azide to a medium.

A New Technique for the Use of Sodium Azide (Hydrazoic Acid) as an Inhibitive Agent1