Open AccessEnumeration of Legionella CFU by Colony Hybridization Using Specific DNA Probes
Author(s) -
Wakako Satoh,
Mayumi Nakata,
Hiroyuki Yamamoto,
Takayuki Ezaki,
Kazumasa Hiramatsu
Publication year - 2002
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.68.12.6466-6470.2002
Subject(s) - enumeration , legionella , biology , dna–dna hybridization , oligonucleotide , dna , microbiology and biotechnology , oligomer restriction , hybridization probe , bacteria , genetics , mathematics , combinatorics
Oligonucleotide probes and colony hybridization (CH) were applied to enumerate organisms of the genus Legionella in cooling tower water. The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one.