Open AccessRapid Approach To Determine rrn Arrangement in Salmonella Serovars
Author(s) -
R. Allen Helm,
Stanley Maloy
Publication year - 2001
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.67.7.3295-3298.2001
Subject(s) - salmonella enterica , salmonella , serotype , biology , polymerase chain reaction , genetics , computational biology , gene , microbiology and biotechnology , bacteria
A PCR method was developed by which to rapidly and accurately determine the rrn arrangement of Salmonella enterica serovars. Primers were designed to the genomic regions flanking each of the seven rrn operons. PCR analysis using combinations of these primers will distinguish each of the possible arrangements of the rrn skeleton.