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The respiration percentage of3 H-labeled glucose by heterotrophic microorganisms in water samples was determined by a method based on equimolar addition of [3 H]- and [14 C]glucose to samples. The respiration percentage of [3 H]glucose exceeded that of [14 C]glucose by 10% over the assayed range of respiration rates. Respiration percentages diminished with decreasing temperature, the relation between respiration of [3 H]- and [14 C]glucose remaining the same. The significance of tritiated water respiration with regard to experimental design is emphasized.

Respiration of Tritiated Substrates in Heterotrophic Activity Assays