Open AccessReplacement of ParC α4 Helix with That of GyrA Increases the Stability and Cytotoxicity of Topoisomerase IV-Quinolone-DNA Ternary Complexes
Author(s) -
Emily S. Pfeiffer,
Hiroshi Hiasa
Publication year - 2004
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.48.2.608-611.2004
Subject(s) - topoisomerase iv , topoisomerase , cytotoxicity , quinolone , escherichia coli , ternary complex , dna , biology , mutant , biochemistry , chemistry , stereochemistry , dna gyrase , enzyme , antibiotics , in vitro , gene
Replacement of the alpha4 helix of ParC with that of GyrA increased the stability of topoisomerase IV-quinolone-DNA ternary complexes. This mutant topoisomerase IV-mediated cell killing was more efficient than topoisomerase IV-mediated cell killing in Escherichia coli. Thus, the alpha4 helix plays critical roles in determining the stability and the cytotoxicity of ternary complexes.