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We isolated 19 lincomycin-resistant Bacillus subtilis mutants by expressing lmrB encoding a putative multidrug efflux protein. Eighteen of the mutants altered at two regions (-3 to -1 and +15) immediately downstream of the -10 region of the lmr promoter increased lmr transcription in vivo and in vitro.

antimicrobial agents and chemotherapy

Lincomycin Resistance Mutations in Two Regions Immediately Downstream of the −10 Region of <i>lmr</i> Promoter Cause Overexpression of a Putative Multidrug Efflux Pump in <i>Bacillus subtilis</i> Mutants

Lincomycin Resistance Mutations in Two Regions Immediately Downstream of the −10 Region of lmr Promoter Cause Overexpression of a Putative Multidrug Efflux Pump in Bacillus subtilis Mutants