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A clinical isolate of Pseudomonas aeruginosa was found to produce a clavulanic acid-inhibited extended-spectrum beta-lactamase with a pI of 6.4. PCR, cloning, and sequencing experiments showed that the corresponding bla(TEM-21) gene was part of a chromosomally located Tn801 transposon disrupted by an IS6100 element and adjacent to an aac(3)-II gene.

antimicrobial agents and chemotherapy

Clinical Strain of Pseudomonas aeruginosa Carrying a bla TEM-21 Gene Located on a Chromosomal Interrupted Tn A Type Transposon