Open Access−11 Mutation in the ampC Promoter Increasing Resistance to β-Lactams in a Clinical Escherichia coli Strain
Author(s) -
Stéphane Corvec,
Nathalie Caroff,
E. Espaze,
Julie Marraillac,
Alain Reynaud
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.10.3265-3267.2002
Subject(s) - escherichia coli , mutation , biology , homology (biology) , gene , strain (injury) , genetics , promoter , microbiology and biotechnology , sequence homology , enterobacteriaceae , peptide sequence , gene expression , anatomy
A mutation was discovered in the Pribnow box of the ampC promoter in a clinical Escherichia coli strain. This -11 C-to-T transition created a perfect homology with the -10 consensus sequence. The new promoter was cloned upstream of the cat gene of pKK232-8 and induced a sixfold increase in promoter strength.