Open AccessMutation of Escherichia coli capable of expressing gene(s) for beta-lactamase production of Citrobacter freundii
Author(s) -
Somay Yamagata Murayama,
Tomoko Yamamoto,
Isamu Suzuki,
Tetsuo Sawai
Publication year - 1986
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.29.4.707
Subject(s) - citrobacter freundii , escherichia coli , gene , mutant , biology , citrobacter , mutation , enterobacteriaceae , genetics , microbiology and biotechnology
A mutation in a chromosomal gene of Escherichia coli, designated reb, acted in trans to increase the expression of the cloned beta-lactamase gene of Citrobacter freundii. The reb gene was located around 99 min. Deletion mutants in the cloned gene(s) which had lost the regulatory region for induction were also constructed.