Open AccessGain- and Loss-of-Function Screens Coupled to Next-Generation Sequencing for Antibiotic Mode of Action and Resistance Studies in Streptococcus pneumoniae
Author(s) -
Hélène Gingras,
Kévin Patron,
Arijit Bhattacharya,
Philippe Leprohon,
Marc Ouellette
Publication year - 2019
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.02381-18
Subject(s) - dihydrofolate reductase , biology , mutagenesis , mode of action , streptococcus pneumoniae , genetics , gene , mutation , atp binding cassette transporter , computational biology , drug resistance , transporter , antibiotics , biochemistry
Two whole-genome screening approaches are described for studying the mode of action and the mechanisms of resistance to trimethoprim (TMP) in the Gram-positiveStreptococcus pneumoniae . The gain-of-function approach (Int-Seq) relies on a genomic library of DNA fragments integrated into a fucose-inducible cassette.
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