Open AccessCytoprotective effects of Morinda officinalis against hydrogen peroxide‐induced oxidative stress in Leydig TM3 cells
Author(s) -
Chang MunSeog,
Kim WonNam,
Yang WoongMo,
Kim HyuYoung,
Oh JiHoon,
Park SeongKyu
Publication year - 2008
Publication title -
asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.1111/j.1745-7262.2008.00414.x
Subject(s) - oxidative stress , catalase , lipid peroxidation , superoxide dismutase , antioxidant , chemistry , leydig cell , hydrogen peroxide , reactive oxygen species , endocrinology , medicine , biochemistry , microbiology and biotechnology , biology , hormone , luteinizing hormone
Abstract Aim: To investigate the antioxidant effects of Morinda officinalis ( Morindae radix , MR) on H 2 O 2 ‐induced oxidative stress in cultured mouse TM3 Leydig cells. Methods: We carried out 2,2‐diphenyl‐1‐picrylhydrazyl free radical scavenging, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase (SOD), and catalase (CAT) assays in Leydig TM3 cells. Results: MR showed a 47.8% 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxidative stress was induced in TM3 cells with 100 μmol H 2 O 2 , and treatment of the cells with 250 μg/mL MR showed the most significant protective effect (64%, P < 0.001) in the cell viability assay with a decreased lipid peroxidation level (1.75 nmol/mg protein, P < 0.05), increased testosterone production (43.5 pg/mL), and improvements in SOD activity (7.49 units of SOD/mg protein, P < 0.001) and C AT activity (74.6 units of CAT/mg protein, P < 0.001). Conclusion: These findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H 2 O 2 ‐induced oxidative stress.