Open AccessKnockdown of Stat3 expression using RNAi inhibits growth of laryngeal tumors in vivo
Author(s) -
GAO Lifang,
Wen Lianji,
YU Hao,
ZHANG Ling,
MENG Yan,
SHAO Yueting,
XU Deqi,
ZHAO Xuejian
Publication year - 2006
Publication title -
acta pharmacologica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.514
H-Index - 90
eISSN - 1745-7254
pISSN - 1671-4083
DOI - 10.1111/j.1745-7254.2006.00277.x
Subject(s) - survivin , cyclin d1 , tunel assay , apoptosis , cancer research , gene knockdown , blot , nude mouse , biology , in vivo , microbiology and biotechnology , transplantation , stat3 , terminal deoxynucleotidyl transferase , chemistry , medicine , cell cycle , gene , biochemistry
Aim : To study the effect of pSilencer 1.0‐U6‐siRNA‐stat3 on the growth of human laryngeal tumors in nude mice. Methods : Hep2 cells were transplanted into nude mice, then at the time of tumor formation, growth rates were observed. After the tumor formed, pSilencer 1.0‐U6‐siRNA‐ stat3 was injected. Tumor volumes were calculated, and growth curves were plotted. Representative histological sections were taken from mice bearing transplantation tumors in both treated and control groups, and start3 , pTyr‐stat3 , Bcl‐2 , cyclin D1 , and survivin expression were detected by Western blotting. survivin mRNA levels were detected by Northern blotting, hematoxylin and eosin staining and terminal deoxyribonucleotidyl transferase‐mediated dUTP‐digoxigenin nick end‐labeling (TUNEL) assay to confirm the apoptosis of tumors. Results : In nude mice, pSilencer 1.0‐U6‐siRNA‐ stat3 significantly suppressed the growth of tumors compared with controls (P < 0.01). In suppressed stat3 expression, and downregulated BcL2, cyclin D1 , and survivin expression within the tumor. This significantly induced apoptosis of the tumors. Conclusion : pSilencer 1.0‐U6‐siRNA‐ stat3 was able to inhibit the growth of transplanted human laryngeal tumors in nude mice and induce apoptosis.