Open AccessExpression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli
Author(s) -
KrauseGruszczynska Malgorzata,
Van Alphen Lieke B.,
Oyarzabal Omar A.,
Alter Thomas,
Hänel Ingrid,
Schliephake Annette,
König Wolfgang,
Van Putten Jos P.M.,
Konkel Michael E.,
Backert Steffen
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2007.00802.x
Subject(s) - campylobacter jejuni , biology , campylobacter , microbiology and biotechnology , escherichia coli , mutant , campylobacter coli , strain (injury) , gene , bacterial outer membrane , bacteria , genetics , anatomy
Abstract Binding of Campylobacter jejuni and Campylobacter coli to host fibronectin is mediated by the 37 kDa outer membrane protein CadF. Immunoblot analysis of 58 C. jejuni and C. coli isolates of human and animal origin showed that CadF is expressed in every strain. In most C. jejuni isolates, a 37 kDa band (p37) and a less‐prominent 32 kDa band (p32) reacted with the antibodies. In C. coli isolates, CadF was consistently larger with sizes of 39 kDa (p39) and 34 kDa (p34), respectively. PCR analysis and sequencing revealed the presence of a 39‐bp insertion sequence in the cadF gene of C. coli strains, explaining the increased molecular size. Infection assays revealed that C. jejuni bound and invaded INT‐407 epithelial cells much more efficiently than C. coli and that this difference was considerably reduced in isogenic cadF mutants. These results demonstrate that CadF is an important pathogenicity factor. The difference between CadF of C. jejuni and C. coli may potentially be exploited to discriminate these species in food and clinical specimens.