Open AccessDiversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize
Author(s) -
Zhang Lixin,
Xie Guanlin
Publication year - 2007
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2006.00530.x
Subject(s) - burkholderia cepacia complex , burkholderia , rhizosphere , biology , burkholderia cenocepacia , microbiology and biotechnology , genetics , bacteria
A survey of Burkholderia cepacia complex (Bcc) species was conducted in agricultural fields within Hangzhou, China. Out of the 251 bacterial isolates recovered on the selective media from the rhizosphere of rice and maize, 112 of them were assigned to Bcc by PCR assays. The species composition of the Bcc isolates was analyzed by a combination of recA ‐restriction fragment length polymorphism assays, species‐specific PCR tests and recA gene sequencing. The results revealed that the majority belong to B. cepacia , Burkholderia cenocepacia recA lineage IIIB, Burkholderia vietnamiensis and Burkholderia pyrrocinia . Burkholderia cenocepacia and B. vietnamiensis dominated the rhizosphere of maize and rice, respectively, indicating that species composition and abundance of Bcc may vary dramatically in different crop rhizospheres. In addition, one isolate (R456) formed a single discrete cluster within the phylogenetic analysis of the Bcc recA gene, and it may belong to a new genomovar.