Open AccessGenetic variability of the frameshift region in IS 911 transposable elements from Escherichia coli clinical isolates
Author(s) -
Licznar Patricia,
Bertrand Claire,
Canal Isabelle,
Prère MarieFrançoise,
Fayet Olivier
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2003.tb11522.x
Subject(s) - frameshift mutation , transposable element , transposition (logic) , escherichia coli , genetics , biology , insertion sequence , mobile genetic elements , dna transposable elements , plasmid , gene , translational frameshift , tn10 , enterobacteriaceae , mutation , mutant , linguistics , philosophy
Abstract The IS 911 bacterial transposable element has been analyzed for its mechanism of transposition and for the way it controls the expression of its genes by programmed −1 translational frameshifting. In the present study the prevalence of IS 911 has been determined in the Enterobacteriaceae family and in other Gram‐negative bacilli. Three variants, found in Escherichia coli clinical isolates and having mutations in the region implicated in frameshifting, were functionally characterized. All three were altered in their frameshifting and transposition abilities, suggesting that the frameshift region of IS 911 may constitute a target for mutations reducing the transposition frequency of this mobile element in natural populations of E. coli .