Open AccessPheno‐ and genotypic properties of streptococci of serological group B of canine and feline origin
Author(s) -
Yildirim Ali Önder,
Lämmler Christoph,
Weiß Reinhard,
Kopp Peter
Publication year - 2002
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2002.tb11265.x
Subject(s) - serology , microbiology and biotechnology , biology , genotype , group a , virology , antibody , immunology , genetics , medicine , gene
Abstract In the present study streptococci of serological group B isolated from canines ( n =48 ) and felines ( n =7 ) were comparatively investigated with group B streptococci from humans and bovines for cultural, biochemical and serological properties for antibiotic resistancies and by molecular analysis. An identification was performed with group B‐specific antiserum, biochemical reactions, by PCR amplification and subsequent endonuclease digestion of the 16S rRNA gene and by amplification of species‐specific parts of the 16S rDNA the 16S–23S rDNA intergenic spacer region and the CAMP factor gene cfb . Phenotypic similarities of group B streptococci of canine and feline origin with group B streptococci from humans and differences to group B streptococci of bovine origin could be observed in lactose fermentation, serotype patterns, pigmentation, growth properties of the bacteria in fluid medium and soft agar, hemagglutination reactions and in minocycline and tetracycline resistance. A negative hyaluronidase plate test, a hyl B amplicon with a size of 4.6 kb and an insertion sequence 1548 could be observed among canine, feline and human group B streptococci of serotype III. The remaining hyaluronidase positive strains, also including all isolates of bovine origin, had a hyl B gene with a size of 3.3 kb. Further genotypic differences could be observed in the occurrence of the genes lmb and scp B which appeared generally among canine, feline and human group B streptococci, but less pronounced among bovine isolates of this species. According to the presented data group B streptococci of canine and feline origin seemed to be more related to human than to bovine isolates of this species possibly indicating some epidemiological relation.