Open AccessListeria monocytogenes response regulators important for stress tolerance and pathogenesis
Author(s) -
Kallipolitis Birgitte H.,
Ingmer Hanne
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10872.x
Subject(s) - listeria monocytogenes , virulence , response regulator , biology , histidine kinase , two component regulatory system , mutant , microbiology and biotechnology , gene , signal transduction , genetics , regulator , transduction (biophysics) , bacteria , biochemistry
Abstract Environmental sensing by two‐component signal transduction systems is likely to play a role for growth and survival of Listeria monocytogenes both during transmission in food products and within a host organism. Two‐component systems typically consist of a membrane‐associated sensor histidine kinase and a gene regulatory protein, the response regulator (RR). We have identified seven putative RR genes in L. monocytogenes LO28 by PCR using degenerate oligonucleotide primers. By insertional inactivation we obtained data suggesting that three of the putative RRs contribute to the pathogenicity of L. monocytogenes in mice. Strikingly, the mutants that were attenuated in virulence also had a decreased ability to grow in the presence of various stress conditions potentially encountered in an infection process. Thus, our data point to a connection between the ability of the putative two‐component systems to sense and respond to certain environmental stimuli, and the virulence of L. monocytogenes .