Open AccessAn enteroaggregative Escherichia coli strain of serotype O111:H12 damages and invades cultured T84 cells and human colonic mucosa
Author(s) -
Abe Cecilia M.,
Knutton Stuart,
Pedroso Margareth Z.,
Freymüller Edna,
Gomes Tânia A.T.
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb10841.x
Subject(s) - enteroaggregative escherichia coli , microbiology and biotechnology , serotype , virulence , strain (injury) , biology , intestinal mucosa , escherichia coli , enterobacteriaceae , cell culture , anatomy , medicine , gene , genetics
Abstract The pathogenic mechanisms of enteroaggregative Escherichia coli (EAEC) are not well defined. We investigated the interaction of EAEC strain 236 (serotype O111:H12) with polarised Caco‐2 and T84 human intestinal epithelial cells lines, and with human jejunal and colonic mucosa. Strain 236 adhered to both polarised cell lines and to both intestinal tissue types, but caused severe damage and was invasive only in T84 cells and colonic mucosa. In contrast, prototype EAEC strain 042, which also adhered to the cultured intestinal cell lines, did not adhere to or invade jejunal or colonic tissue. These observations suggest a heterogeneity of virulence properties within the EAEC category of diarrhoea‐causing E. coli .