Open AccessGreen fluorescent protein‐tagged Edwardsiella tarda reveals portal of entry in fish
Author(s) -
Ling Sharon H.M,
Wang Xian Hui,
Lim Tit Meng,
Leung Ka Yin
Publication year - 2001
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.2001.tb09476.x
Subject(s) - edwardsiella tarda , gill , biology , virulence , gastrointestinal tract , microbiology and biotechnology , pathogen , population , green fluorescent protein , fish <actinopterygii> , fishery , gene , biochemistry , demography , sociology
Abstract The application of green fluorescent protein (GFP) to identify the portal(s) of entry of bacterial pathogens in animal hosts was studied using the fish pathogen Edwardsiella tarda and blue gourami, Trichogaster trichopterus . An immersion challenge model was utilized to mimic natural infection conditions in fish. Gastrointestinal tract, gills and the body surface of fish were found to be the sites of entry of virulent E. tarda (PPD130/91) by histological and infection kinetics studies. On the other hand, avirulent E. tarda (PPD125/87) was mainly found in the gastrointestinal tract, and the bacterial population in tissue declined over a period of 7 days.