Open AccessIdentification of the genes encoding enzymes involved in the early biosynthetic pathway of pteridines in Synechocystis sp. PCC 6803
Author(s) -
Woong Lee Soo,
Woo Lee Hee,
Jae Chung Hyun,
Kim YoungA,
Jeong Kim Yeon,
Hahn Yoonsoo,
Hoon Chung Jae,
Shik Park Young
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13658.x
Subject(s) - open reading frame , synechocystis , biochemistry , biology , escherichia coli , gene , enzyme , atp synthase , biosynthesis , aldolase a , peptide sequence , mutant
Abstract The biosynthetic pathway for the pteridine moiety of cyanopterine, as well as tetrahydrobiopterine, has been investigated in Synechocystis sp. PCC 6803. Open reading frames slr0426, slr1626, slr0078 and sll0330 of the organism putatively encoding GTP cyclohydrolase I, dihydroneopterine aldolase, 6‐pyruvoyltetrahydropterine synthase and sepiapterine reductase, respectively, have been cloned into T7‐based vectors for expression in Escherichia coli . The recombinant proteins have been purified to homogeneity and demonstrated to possess expected genuine activities except that of sll0330. Our result is the first direct evidence for the functional assignment of the open reading frames in Synechocystis sp. PCC 6803. Furthermore, the 6‐pyruvoyltetrahydropterine synthase gene is demonstrated for the first time in prokaryotes. Based on the result, biosynthesis of cyanopterine is discussed.