Open AccessConstruction of a new cloning vector utilizing a cryptic plasmid and the highly expressed melanin‐synthesizing gene operon from Streptomyces castaneoglobisporus
Author(s) -
Ikeda Kayo,
Suzuki Koji,
Yoshioka Hideki,
Miyamoto Katsushiro,
Masujima Tsutomu,
Sugiyama Masanori
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13273.x
Subject(s) - thiostrepton , biology , streptomyces , plasmid , cloning vector , cloning (programming) , operon , molecular cloning , genetics , multiple cloning site , gene , streptomycetaceae , vector (molecular biology) , microbiology and biotechnology , gene expression , escherichia coli , actinomycetales , recombinant dna , bacteria , rna , computer science , programming language , ribosome
Abstract Streptomyces castaneoglobisporus HUT6202 overproduces a diffusible melanin pigment and harbors a cryptic 7.4‐kb plasmid, pHY6202. We constructed a Streptomyces cloning vector, pSY10CMM, consisting of the HUT6202 rep gene, the thiostrepton resistance gene and an operon encoding the synthesis of melanin pigment, abbreviated mel , from S. castaneoglobisporus . The vector, which has Sph I and Bam HI sites as cloning sites with insertional inactivation of the mel , is a more convenient cloning vector than commonly used pIJ702, because of its broad host range for antibiotic‐producing Streptomyces strains and its much greater production of diffusible melanin pigment.