Open AccessEngineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini‐Tn 5 constructs
Author(s) -
Winson Michael K,
Swift Simon,
Hill Philip J,
Sims Catriona M,
Griesmayr Gottfried,
Bycroft Barrie W,
Williams Paul,
Stewart Gordon S.A.B
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13045.x
Subject(s) - bioreporter , photorhabdus luminescens , biology , plasmid , photorhabdus , gene cassette , reporter gene , operon , transposable element , genetics , bioluminescence , gene , mutant , gene expression , ecology , integron
Abstract The luxCDABE operon of Photorhabdus luminescens has been cloned and engineered as an easily mobilisable cassette flanked by sites for commonly used restriction enzymes. Constitutive and promoter probe plasmids utilising the P. luminescens luxCDABE have been constructed using a number of compatible replicons and antibiotic markers. Complementary to these plasmids, a range of promoterless and constitutive luxCDABE mini‐Tn 5 derivatives has been constructed. The potential of coupling mini‐Tn 5 luxCDABE promoter probe transposons with automated luminometry and photometry to screen for mutants that exhibit growth phase variation in gene expression is demonstrated.