Open AccessExpression of plasma membrane proton‐ATPase gene in salt‐tolerant yeast Zygosaccharomyces rouxii is induced by sodium chloride
Author(s) -
Watanabe Yasuo,
Sanemitsu Yumi,
Tamai Youichi
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06558.x
Subject(s) - yeast , atpase , sodium , biochemistry , northern blot , membrane , saccharomyces cerevisiae , western blot , chemistry , biology , messenger rna , microbiology and biotechnology , gene , enzyme , organic chemistry
Abstract The amounts of mRNA and protein of plasma membrane proton‐ATPase were measured in the salt‐tolerant yeast Zygosaccharomyces rouxii by Northern and Western blot analyses. Although their amounts were independent of growth phase, their synthesis were induced when yeast cells were grown in the presence of NaCl or were subjected to NaCl shock. This finding was consistent with our previous result that plasma membrane proton‐ATPase activity was elevated in Z. rouxii cells grown in medium containing high concentrations of NaCl.