Open AccessEffect of downstream message secondary structure on the secretory expression of the Streptomyces subtilisin inhibitor
Author(s) -
Taguchi Seiichi,
Yoshida Yasuto,
Kumagai Izumi,
Miura Kinichiro,
Momose Haruo
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06028.x
Subject(s) - gene , subtilisin , streptomyces , inverted repeat , biology , downstream processing , downstream (manufacturing) , start codon , genetics , ribosomal binding site , streptomycetaceae , microbiology and biotechnology , actinomycetales , bacteria , messenger rna , biochemistry , translation (biology) , operations management , genome , economics , enzyme
Abstract The contribution of downstream message secondary structure to the production of Streptomyces subtilisin inhibitor (SSI) was investigated in Streptomyces lividans 66 using a cloned gene. By deletion analysis, two inverted repeats located downstream from the stop codon in the SSI gene were found to exhibit a marked effect on the amount and homogeneity of SSI gene transcript and, consequently, the efficiency of SSI productivity.