Open AccessTranscription in vitro and in vivo of the 7S RNA gene associated with the ribosomal RNA operon in the hyperthermophilic archaeon Methanothermus fervidus
Author(s) -
Koller Gabriele,
Reeve John N.,
Frey Gerhard,
Thomm Michael
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05496.x
Subject(s) - operon , rna , transcription (linguistics) , biology , gene , ribosomal rna , microbiology and biotechnology , transfer rna , methanococcus , biochemistry , archaea , escherichia coli , linguistics , philosophy
Abstract The gene encoding the archaeal 7S RNA in the hyperthermophile Methanothermus fervidus is linked to a tRNA Ser and rRNA operon in the arrangement 5′‐7S RNA‐14nt‐tRNA Ser ‐196nt‐16S rRNA and the promoter directing transcription of this 7S RNA gene has now been identified. Initiation of transcription of the 7S RNA gene has been shown to occur both in vivo in M. fervidus and in vitro, using a Methanococcus thermolithotrophicus derived cell‐free transcription system, at the first G residue within the initiator sequence ATGG, located 6 bp upstream of the 5′ end of the 7S RNA coding region. Cotranscription of the 7S RNA and tRNA Ser has been demonstrated in vitro.