Open AccessCloning and expression of an extracellular α‐amylase gene from Streptomyces hygroscopicus in Streptomyces lividans 66
Author(s) -
McKillop Christine,
Elvin Peter,
Kenten John
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01656.x
Subject(s) - streptomyces hygroscopicus , subcloning , biology , microbiology and biotechnology , streptomyces , gene , molecular cloning , plasmid , gene expression , genetics , bacteria
Abstract An extracellular α‐amylase gene was cloned from Streptomyces hygroscopicus into Streptomyces lividans 66 using the multicopy vector pIJ702. Subcloning of DNA from the original clone allowed the gene to be localized to a 2.8 kb segment of DNA. Use of a promoter probe vector gave a possible location of the gene promoter and also the direction of transcription of the gene. The α‐amylase protein was identified as a 48‐kDal band after sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE). Increased levels of this protein were produced and secreted into the medium by the clone compared to S. hygroscopicus .