Open AccessNitrogenase activity (acetylene reduction) in root‐associated, cold‐climate species of Azospirillum, Enterobacter, Klebsiella and Pseudomonas growing at various temperatures
Author(s) -
Haahtela Kielo
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01151.x
Subject(s) - nitrogenase , diazotroph , enterobacter , klebsiella pneumoniae , klebsiella , microbiology and biotechnology , pseudomonas , klebsiella oxytoca , biology , bacteria , nitrogen fixation , biochemistry , escherichia coli , gene , genetics
Abstract 23 Strains of diazotrophic root‐associated bacteria isolated from various parts of Finland were tested for nitrogenase activity during growth at various temperatures. Nitrogenase activity was optimal at 20–37°C in cultures of Klebsiella pneumoniae , and at 14–20°C in cultures of Klebsiella terrigena and Enterobacter agglomerans . Strains of K. terrigena and E. agglomerans showed no activity at 37°C, and K. pneumoniae only minimal or no activity at 14°C. Azospirillum lipoferum exhibited high nitrogenase activity at both 28–37°C, but less than 25% of optimal activity at 20°C and no activity at 14°C. Pseudomonas sp. expressed nitrogenase activity at 14–28°C. None of the strains manifested nitrogenase activity at 4 or 42°C. There were only small local variations within a species between strains isolated at different locations.