Open AccessInduction of proteins during phage reactivation induced by UV irradiation, oxygen and peroxide in Bacteroides fragilis
Author(s) -
Schumann J.Petra,
Jones David T.,
Woods David R.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb01048.x
Subject(s) - bacteroides fragilis , bacteriophage , gel electrophoresis , polyacrylamide gel electrophoresis , microbiology and biotechnology , chemistry , sodium dodecyl sulfate , peroxide , biology , biochemistry , escherichia coli , enzyme , gene , organic chemistry , antibiotics
Abstract Phage reactivation systems in Bacteroides fragilis were induced by far‐UV irradiation, O 2 and H 2 O 2 . These three treatments also induced the synthesis of 3, 6, and 4 protein bands, respectively, which were easily detectable by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE). Two proteins with apparent M r s of approx. 90 000 and 70 000 were induced by all three treatments. Caffeine completely inhibited UV‐ and O 2 ‐induced phage reactivation and prevented the synthesis of the M r 90 000 and M r 70 000 proteins. The results suggest that these two proteins may be involved in phage reactivation processes induced by UV, O 2 and H 2 O 2 in B. fragilis .