Open AccessRabbit enterotoxaemia: Purification and preliminary characterisation of a toxin produced by Clostridium spiroforme
Author(s) -
Fernie D.S.,
Knights J.M.,
Thomson R.O.,
Carman R.J.
Publication year - 1984
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1984.tb00212.x
Subject(s) - antitoxin , clostridium perfringens , toxin , size exclusion chromatography , clostridium , chromatography , ultrafiltration (renal) , hela , microbiology and biotechnology , chemistry , ion chromatography , biology , biochemistry , bacteria , in vitro , genetics , enzyme
Abstract A culture filtrate of Clostridium spiroforme grown in a brain‐heart‐infusion peptone medium at 37°C for 24 h was concentrated by ultrafiltration, and the toxic fraction separated by ion exchange chromatography. This material was dermonecrotic in depilated guinea pigs, lethal in mice, enterotoxic in rabbit ileal loops and infant mice and cytophathic for HeLa cells. All activity was neutralised by Clostridium perfringens Type E antitoxin. Analysis by size exclusion high‐performance liquid chromatography produced a single symmetrical peak corresponding to an M r of 41 000 to 42 000.