Open AccessVariations among clinical isolates of Staphylococcus aureus to induce expression of E‐selectin and ICAM‐1 in human endothelial cells
Author(s) -
Strindhall Jan,
Lindgren PerEric,
Löfgren Sture,
Kihlström Erik
Publication year - 2002
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2002.tb00558.x
Subject(s) - staphylococcus aureus , microbiology and biotechnology , biology , e selectin , cell adhesion molecule , intercellular adhesion molecule 1 , icam 1 , bacteria , cell adhesion , cell , immunology , biochemistry , genetics
Abstract Eighteen clinical isolates of Staphylococcus aureus , nine methicillin‐sensitive and nine methicillin‐resistant, were investigated for their ability to induce expression of E‐selectin and ICAM‐1 in human endothelial cells. Upregulation of adhesion molecules varied between isolates; 17 isolates induced expression of E‐selectin and 13 of ICAM‐1. Some isolates induced a significant expression of E‐selectin without stimulation of ICAM‐1, whereas the opposite was not found. Bacterial viability was required for induction of the adhesion molecules. The kinetics of ICAM‐1 expression in S. aureus ‐infected cells differed from those stimulated with interleukin‐1β (IL‐1β). On the other hand, expression of E‐selectin was very similar in S. aureus ‐infected and IL‐1β‐stimulated cells. There was no correlation between ability of S. aureus to induce expression of cell adhesion molecules, methicillin susceptibility, pulse field gel electrophoresis patterns, biochemical characteristics, phage typing and toxin production.