Open AccessQuorum quenching in cultivable bacteria from dense marine coastal microbial communities
Author(s) -
Romero Manuel,
MartinCuadrado AnaBelen,
RocaRivada Arturo,
Cabello Ana María,
Otero Ana
Publication year - 2011
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2010.01011.x
Subject(s) - biology , quorum sensing , quorum quenching , actinobacteria , chromobacterium violaceum , gammaproteobacteria , bacteroidetes , marine bacteriophage , bacteria , microbiology and biotechnology , firmicutes , roseobacter , homoserine , alteromonas , photobacterium , vibrio , ecology , biofilm , 16s ribosomal rna , phylogenetics , biochemistry , clade , gene , genetics
Abstract Acylhomoserine lactone (AHLs)‐mediated quorum‐sensing (QS) processes seem to be common in the marine environment and among marine pathogenic bacteria, but no data are available on the prevalence of bacteria capable of interfering with QS in the sea, a process that has been generally termed ‘quorum quenching’ (QQ). One hundred and sixty‐six strains isolated from different marine dense microbial communities were screened for their ability to interfere with AHL activity. Twenty‐four strains (14.4%) were able to eliminate or significantly reduce N ‐hexanoyl‐ l ‐homoserine lactone activity as detected by the biosensor strain Chromobacterium violaceum CV026, a much higher percentage than that reported for soil isolates, which reinforces the ecological role of QS and QQ in the marine environment. Among these, 15 strains were also able to inhibit N ‐decanoyl‐ l ‐homoserine lactone activity and all of them were confirmed to enzymatically inactivate the AHL signals by HPLC‐MS. Active isolates belonged to nine different genera of prevalently or exclusively marine origin, including members of the Alpha ‐ and Gammaproteobacteria (8), Actinobacteria (2), Firmicutes (4) and Bacteroidetes (1). Whether the high frequency and diversity of cultivable bacteria with QQ activity found in near‐shore marine isolates reflects their prevalence among pelagic marine bacterial communities deserves further investigation in order to understand the ecological importance of AHL‐mediated QS and QQ processes in the marine environment.