Open AccessIdentification and characterization of a novel inhibitor of alginate overproduction in Pseudomonas aeruginosa
Author(s) -
Withers T. Ryan,
Yin Yeshi,
Yu Hongwei D.
Publication year - 2014
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12102
Subject(s) - overproduction , cosmid , pseudomonas aeruginosa , complementation , transposon mutagenesis , biology , transposable element , protein fragment complementation assay , locus (genetics) , mutant , gene , mutagenesis , microbiology and biotechnology , genetics , bacteria
In this study, we performed whole‐genome complementation using a PAO 1‐derived cosmid library, coupled with in vitro transposon mutagenesis, to identify gene locus PA 1494 as a novel inhibitor of alginate overproduction in P. aeruginosa strains possessing a wild‐type mucA .