Open AccessDraft genome sequences of five recent human uropathogenic E scherichia coli isolates
Author(s) -
Subashchandrabose Sargurunathan,
Hazen Tracy H.,
Rasko David A.,
Mobley Harry L. T.
Publication year - 2013
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12059
Subject(s) - biology , escherichia coli , genome , gene , microbiology and biotechnology , phylogenetic tree , comparative genomics , pathogenicity island , genetics , gene cluster , genomics
This study reports the release of draft genome sequences of five isolates of uropathogenic E scherichia coli ( UPEC ), isolated from patients suffering from uncomplicated cystitis in 2012 in A nn A rbor, M ichigan. Phylogenetic analyses revealed that these strains belonged to E . coli phylogroups B 2 and D and are closely related to known UPEC strains. Comparative genomic analysis revealed that more conserved proteins were shared between these recent isolates and UPEC strains causing cystitis than those causing pyelonephritis. Additional genomic comparisons identified that three isolates encode a type III secretion system ( T 3 SS ) and a putative T 3 SS effector gene cluster along with an invasin‐like outer membrane protein. The presence of T 3 SS genes is a rare occurrence among UPEC strains. These genomes further substantiate the heterogeneity of the gene pool of UPEC and provide a foundation for comparative genomic studies using recent clinical isolates.