Open AccessMolecular characterization of S accharomyces cerevisiae α‐pheromone by mass spectrometry‐based peptidomics
Author(s) -
Bener Aksam Eda,
Pinkse Martijn W.H.,
Verhaert Peter D.E.M.
Publication year - 2013
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/1567-1364.12032
Subject(s) - pheromone , yeast , biology , saccharomyces cerevisiae , mass spectrometry , sex pheromone , peptide , biochemistry , computational biology , microbiology and biotechnology , chromatography , genetics , chemistry
Using modern peptide analytical MS technology (‘Peptidomics’), it is possible to analyze yeast α‐pheromone both qualitatively and semi‐quantitatively directly from conditioned cell culture media. MS / MS analysis shows both forms of α‐pheromone ( MF α and MF α') detectable and identifiable straight from WT supernatants. In addition to the mature intact α‐pheromones, also post‐translationally modified α‐pheromone peptides and fragments thereof are found to be present in the culture medium. This molecular analytical technique is complementary to the recently described quantitation method by Rogers et al . (2012, FEMS Yeast Res . 12:668) based on ELISA .