Open AccessCrystallization and X‐ray structure determination of a thermoalkalophilic lipase from Geobacillus SBS‐4S
Author(s) -
Tayyab Muhammad,
Rashid Naeem,
Angkawidjaja Clement,
Kanaya Shigenori,
Akhtar Muhammad
Publication year - 2013
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309113004910
Subject(s) - orthorhombic crystal system , lipase , crystallization , geobacillus stearothermophilus , crystallography , hydrolysis , materials science , resolution (logic) , chemistry , thermophile , chromatography , analytical chemistry (journal) , crystal structure , enzyme , biochemistry , organic chemistry , artificial intelligence , computer science
A thermoalkalophilic lipase (LIP SBS ) from the newly isolated Geobacillus strain SBS‐4S which hydrolyzes a wide range of fatty acids has been characterized. In the present study, the crystallization of purified LIP SBS using the sitting‐drop vapour‐diffusion method and its X‐ray diffraction studies are described. The crystals belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 55.13, b = 71.75, c = 126.26 Å. The structure was determined at 1.6 Å resolution by the molecular‐replacement method using the lipase from G. stearothermophilus L1 as a model.