Open AccessExpression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris
Author(s) -
Jacobs Melisa,
Salinas Silvina R.,
Bianco María I.,
Ielpi Luis
Publication year - 2012
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309112036597
Subject(s) - xanthomonas campestris , crystallization , xanthan gum , secretion , bacterial outer membrane , membrane , resolution (logic) , chemistry , xanthomonas , rhamnose , biochemistry , polysaccharide , rheology , materials science , organic chemistry , escherichia coli , gene , artificial intelligence , computer science , composite material
GumB is a predicted outer membrane lipoprotein that is involved in the synthesis and/or secretion of xanthan gum. This exopolysaccharide, produced by Xanthomonas campestris , is valuable in industry because of its important rheological properties. Solution of the GumB structure will provide insight into the polymerization and/or secretion mechanisms of xanthan gum. GumB was overexpressed and purified and diffraction‐quality crystals of native GumB were obtained. A complete data set was collected to 2.54 Å resolution with an R p.i.m. of 0.034. The crystals belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 84.4, b = 90.5, c = 120.7 Å.