Open AccessCrystallization and X‐ray data collection of HP0902 from Helicobacter pylori 26695
Author(s) -
Sim DaeWon,
Song Jung Hyun,
Lee Woo Cheol,
Lee YooSup,
Kim HyeYeon,
Won HyungSik
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309111039315
Subject(s) - helicobacter pylori , resolution (logic) , recombinant dna , crystallization , crystallography , crystal (programming language) , strain (injury) , diffraction , biology , chemistry , physics , biochemistry , genetics , optics , gene , computer science , organic chemistry , anatomy , artificial intelligence , programming language
HP0902 from Helicobacter pylori 26695 belongs to the cupin superfamily of proteins, which encompasses proteins with a great diversity in function. In this work, two types of recombinant HP0902 protein were crystallized: one with an N‐terminal His 6 tag ( H6 HP0902) and the other with a C‐terminal His 6 tag (HP0902 H6 ). The H6 HP0902 crystal diffracted to 1.40 Å resolution and belonged to space group P 2 1 , with unit‐cell parameters a = 33.5, b = 78.6, c = 41.4 Å. The HP0902 H6 crystal belonged to space group P 4 3 2 1 2 or P 4 1 2 1 2 and diffracted to 2.5 Å resolution, with unit‐cell parameters a = b = 50.4, c = 142.0 Å.