Open AccessCrystallization and preliminary X‐ray crystallographic study of the human MST2 SARAH domain
Author(s) -
Song Jinsue,
Hong Hyerim,
Choi Saehae,
Lee Yong Hee,
Yamashita Eiki,
Bae SukChul,
Park Il Yeong,
Lee Soo Jae
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309111033823
Subject(s) - crystallization , crystallography , resolution (logic) , domain (mathematical analysis) , crystal (programming language) , diffraction , chemistry , materials science , physics , optics , mathematics , artificial intelligence , computer science , programming language , mathematical analysis , organic chemistry
The SARAH domain at the C‐terminus of human MST2 (residues 436–484) was overproduced and purified using an Escherichia coli expression system. The purified domain was crystallized using the hanging‐drop vapour‐diffusion technique. Two crystal forms were obtained. The crystals belonged to space group P 2, with unit‐cell parameters a = 62.0, b = 119.2, c = 62.0 Å, α = 90.0, β = 90.5, γ = 90.0°, or to space group P 6 1 22, with unit‐cell parameters a = 54.5, b = 54.5, c = 303.1 Å. These crystals diffracted to 2.7 and 3.0 Å resolution, respectively.